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Multiplex Serosurveillance

These modules provide an overview of multiplex serosurveillance and offers resources on study design, laboratory methods, data analysis, and translating results into programmatic action. These modules are intended for stakeholders such as policymakers, epidemiologists, surveillance officers, laboratory personnel or others interested in conducting multiplex serosurveillance. Each page includes references and helpful materials for additional information.  

This module provides an overview of what multiplex serosurveillance is, its benefits, use cases, and its competitive advantage in informing public health decisions. 

 

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This module outlines how to design a multipathogen serosurvey, including pathogen selection, sampling, and survey strategy. 

 

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This module covers practical aspects of multiplex assay development, instrument selection, validation, and lab QC. 

 

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This section covers how to process, analyze, and interpret multiplex serosurveillance data, including establishing cutoffs for seropositivity, combining results across multiple antigens, and integrating metadata to generate actionable insights. 

 

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This module provides guidance on interpreting and triangulating multiplex data to inform public health programs and policy. 

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Building Momentum for Integrated Multipathogen Serosurveillance: A Roadmap for a Possible Route to 2030

This living roadmap — co-created by a global group of epidemiology, laboratory, and implementation experts — outlines the opportunities, barriers, and essential milestones to expanding multiplex serosurveillance use in low- and middle-income country public health systems. This report seeks to equip serosurveillance stakeholders with a framework and resource to help develop their own targeted, resourced strategies with goals, activities, and metrics suited to actual funding and capacity.

View the roadmap here